Detection of porcine species DNA on meat processed food samples (shredded meat) using Real-Time PCR

DNA detection of porcine species in processed meat samples (shredded meat) was carried out as an effort to enrich the literature and reference alternative testing methods control halal products circulating Indonesia. The purpose this study detect using real-time PCR. research method used is a qualitative technique. This technique analyzes CT value sample compared positive by looking at formation sigmoid curve if amplification occurs. controls consisted negative control, other LOD control. extraction column centrifuge results were then measured nano photometer see purity concentration extracted DNA. Based on research, it known that read nanophotometer wavelength A260 / A280 show 24,350 with 21,164. For testing, PCR analysis showed detected 39.77, 31.66, 28.32. for specificity not detected. conclusion can be presence